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Description

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When using time for code selection, 20-29 minutes of total time is spent on the date of the encounter 99214 Office or other outpatient visit for the evaluation and management of an established patient, which requires a medically appropriate history and/or examination and moderate level of medical decision making

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To overcome this limitation, we adapted our cell-based autoprocessing assay for high-throughput screening (HTS) using an amplified luminescent proximity homogeneous assay ELISA (AlphaLISA) to quantify autoprocessing efficiency 41

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Limited proteolysis was performed by enzymatic digestion at 37 C adding bovine trypsin (Sigma-Aldrich, Italy) or proteinase K (Sigma-Aldrich, Italy) to protein extract at a ratio 1:100 enzyme/substrate (w/w)

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To reduce intracellular Fe 3+ levels and prevent its conversion to Fe 2+ , iron chelators are widely employed

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