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To prevent ethanol evaporation, the cell incubators were equilibrated with 100 mM EtOH in the humidification pan

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In brief, HEK293T cells (American Type Culture Collection (ATCC), CRL-3216) were cultured in complete DMEM (Gibco, 11965-092) supplemented with 10% (v/v) FBS (VWR, 97068-085) and 1% penicillinstreptomycin (VWR, 16777-164) at 37 C under 5% CO 2

These include nausea, bruising, mild headaches, numbness, skin rashes and chest pains