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Vitamin K protects tissues from ferroptosis We then tested whether vitamin K can also prevent ferroptosis in vivo using mice with genetic deletion of Gpx4 and pathological models, in which ferroptosis results in tissue injury

In contrast, TIGAR overexpression or treatment with Fer-1, a ferroptosis inhibitor, significantly increases NADPH, GPX4, and GSH levels, reduces GSSG expression, and attenuates neuronal ferroptosis and neurological impairment

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Cell cycle analysis The position of the cells in the cell cycle was determined by flow cytometric analysis of the DNA content using propidium iodide