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Mol Med 22:694704 Sitkovsky M, Ohta A (2013) Targeting the hypoxia-adenosinergic signaling pathway to improve the adoptive immunotherapy of cancer

As with the alamarBlue assay, 3D cultures were treated with the insult (H 2 O 2 ) and then with individual B vitamins or the combination of all three neurotropic B vitamins, then incubated for 6 h before adding 1 L NeuroFluor to each well of the 96-well plate