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Mistake 5: using expired medication The expiration date assumes continuous refrigeration

There is also an often cited Japanese study that made patch tests with lavender oil for 9 years and found a huge increase in lavender oil sensitivity in 1997 (from 1.1% in 1990 to 8.7% in 1997 and 13.9% in 1998)

In patients with active IBD, pro-inflammatory cytokines such as TNF-, IL-1, IL-6, IL-12, and IL-23 are significantly elevated, while anti-inflammatory cytokines such as IL-10 and IL-4 are expressed at insufficient levels [59, 60]

The remaining library preparation and sequencing followed the same protocols described for barcode sequencing, with custom indices for the second-round PCR detailed in Supplementary Table 3