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GSH glutathione, NAPQI N-acetyl-p-benzoquinone imine (Reproduced with permission from Vessey [20]) 6 Hepatic Bilirubin Metabolism Bilirubin is a pigment derived from the catabolism of heme

doi: 10.1017/s1092852924000518 34 KhavariBMahmoudiEGeaghanMPCairnsMJ

The first step (0.74%) occurred below 100 C and corresponded to the loss of physically adsorbed water and free guest water

Assays to test the influence of higher temperatures on the substrate preferences were performed at 70 C in SAH/SIH synthesis and cleavage directions with two selected model enzymes, Pfu SAHH (Euryarcheota) and Sac SAHH (Crenarchaeota)