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*Correspondence: Giovanna Mantovani, [email protected] Specialty section: This article was submitted to Systems and Translational Endocrinology, a section of the journal Frontiers in Endocrinology Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

10.1073/pnas.90.2.567 5 AsthanaS.MallickB.AlexandrescuA

Dephosphorylated RNAs were then immediately radiolabelled using T4 PNK and [- 32 P] ATP in rCutSmart buffer (NEB, B6004S) supplemented with 5 mM DTT (Invitrogen, 15508013) at 37 C for 12 h, then desalted using Micro Bio-Spin P-30 columns (Bio-Rad, 7326250), Micro Bio-Spin P-6 columns or Amersham MicroSpin G-25 columns (Cytiva, 27532501) and gel-purified on a urea-polyacrylamide gel

Am J Nephrol 46:7381

Molecular dynamic simulations of oxidized skin lipid bilayer and permeability of reactive oxygen species