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Intracellular zinc homeostasis and zinc signaling

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By contrast, the expression of fatty acid synthase ( Fas ) in liver, for example, was not decreased in Insr P1195L/ +/HFD mice (Supplementary Fig

Abbreviations: ADP: Adenosine diphosphate ATP: Adenosine triphosphate BSA: Bovine serum albumin DMEM: Dulbecco's modified Eagle's medium DMSO: Dimethylsulfoxide DTT: Dithiothreitol CHAPS: 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate EDTA: Ethylenediaminetetraacetic acid EGTA: Ethylene glycol tetraacetic acid FACS: Fluorescence-activated cell sorting FBS: Fetal bovine serum HBSS: Hank's Balanced Salt Solution HEPES: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid HTRF: Homogeneous Time Resolved Fluorescence MIP1a: Macrophage Inflammatory Protein form 1a (also known as CCL3) PBS: phosphate-buffered saline RPMI: Roswell Park Memorial Institute medium TR-FRET: Time-Resolved Fluorescence Resonance Energy Transfer Kinase Glo Luminescent Kinase Assay (Kglo) for PI 3-kinase alpha (A), PI 3-kinase beta (B), Vps34 (C), PI 4-kinase beta (D) The luminescence-based ATP detection reagent KinaseGlo was obtained from Promega, (Cat

S.PaniniP.et al (2014)