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At t = 0 min, 1 ml of bacterial culture ( oxyR-msrA , oxyR-msrB or no plasmid) was mixed with 1 ml of NFs and incubated at 37 C, 160 rpm

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(1988) characterized IGFBP binding determinants in the IGF-1 molecule, providing the structural basis for the R3 substitution that became the foundation of the LR3 design (PMID 3356790)