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E14.5 and P0 cortical tissue was shredded using Ultra-Turrax in 1 mL Trizol

This distinction matters because exogenous GH administration carries a different risk profile than peptide-stimulated endogenous release

No obvious cytotoxicity was observed when the AC16 cells were treated with a high concentration of TP-10@CaP-CTP nanoparticles (200 g/mL), as shown in Supplementary Fig