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In vitro transcribed (ivt) RNAs corresponding to these variants were transfected into Huh7-Lunet CD81 high cells, and luciferase activity in lysates was quantified as a correlate of RNA replication

The following day eyes were incubated in tyrosine hydroxylase antibody (Millipore) (1:200) in permeabilisation buffer for 4 h at 37 C in a humid chamber

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