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The supernatants were collected by centrifugation at 5000 rpm for 5 min at 4 C and precleared by incubated with GFP-Trap agarose beads (Chromotek) or HA-Beads (Roche) for 2 h at 4 C with rotation

Catalase (CAT) activity was assayed in both liver and testis homogenates by the method of Luck [39]

The second step was the introduction of the enzyme collagenase into the liver lobes disrupting the supporting extracellular matrix

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