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The XenoMouse strains were engineered with yeast artificial chromosomes (YACs) containing 245 kb and 190 kb-sized germline configuration fragments of the human heavy chain locus and kappa light chain locus, respectively, which contained core variable and constant region sequences
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Additionally, in FDX1 knockout experiments, there was a significant decrease in protein lipoylation of DLAT and another TCA cycle component called dihydrolipoamide S-succinyltransferase (DLST), both of which no longer bound copper, suggesting the necessity of lipoyl moiety for copper binding
