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Samples were then mixed with an equal volume of metaphosphoric acid (10 g per 100 ml) and centrifuged at 580 g (2,500 r.p.m.) at 4 C for 5 min to precipitate protein

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Although the overall eligibility range was 112 years, all participants who completed the dietary intervention and were included in the prepost metabolomic analyses were aged 4 years (range: 49 years

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