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Npu DnaE intein with Asp118Gly mutation To test the effect of the D118G mutation, the amino acid substitution was introduced into C-GFP via site directed mutagenesis to form C*-GFP (construct 3)

doi: 10.3389/fphar.2026.1801474 Received 01 February 2026 Revised 27 April 2026 Accepted 28 April 2026 Published 19 May 2026 Volume 17 - 2026 Edited by Sarmistha Saha, GLA University, India Reviewed by Aron Weller, Bar-Ilan University, Israel Muhammad Asim, Stanford University, United States Updates Copyright 2026 Pilatti, Philippsen, Bertollo, Webler, Bordignon and Igncio

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