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10.1002/jcp.30988 168 WangJ.CuiY.WangM.MaW.WangL.YuS.et al (2024a)

Detergent was removed by three successive additions of Bio-Beads SM-2 (Bio-Rad)

In line with this, activation of the Nrf2-antioxidant response element (ARE)[99] oxidative stress responsive system occurs in distal muscles prior to the disease onset in mutSOD1 mice[100], supporting the hypothesis that increased muscle oxidative stress is involved in an early phases of ALS, eventually followed by axonal dying back and culminating with loss of motor neurons