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The culture was then centrifuged at 4C and 6,000 rpm for 10 min, and the bacterial pellet was washed twice with phosphate-buffered saline (PBS)

( E ) Immunoblotting of xCT, GPX4 and -actin in Pfa1 and xCT OE cells, 24 h (left panel) or 48 h (right panel) after treatment with Tam (0.75 M) and/or Lipr (1 M)

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one member of the ACC synthase gene family (ACS6) (Garca et al

In some cases, the scFv is expressed as a fusion protein linked to a viral coat protein (e.g., the N-terminus of the viral pIII coat protein)