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After the treatment, 20 L of reagent was added to a 96-well plate containing the cell suspensions, and the plate was incubated for 4 hours

Accessed 20 March 2022 Polegato BF, Pereira AG, Azevedo PS, Costa NA, Zornoff LAM, Paiva SAR, Minicucci MF (2019) Role of thiamine in health and disease

Yeast strains The following yeast strains were used: SP4, a wild-type strain with the genotype MAT, leu1, arg4 70 , referred to here as wt1 DSCD1-1C, a mutant derived from the parent strain SP4 with the genotype MAT, leu1, arg4, sod1::natMX 25 , referred to as sod1 EG103, a wild-type strain with the genotype MAT leu2-3,112 his3l trp1-289 ura3-52 , referred to here as wt2, and its derivative strains containing coding region insertions TRP1 in EG110 ( sod2 ::TRP1), referred to as sod2

duttonii Ly (93.6 to 99.6%) (Fig