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Metabolic activity assays in intact RBCs Washed RBCs were prepared in isotonic HEPES buffer containing either (i) 5 mM [U 13 C]-glucose (Isotec), (ii) 5 mM glucose, 5 mM alanine, and 5 mM [ 13 C 1,2,3,4 ]--ketoglutarate (Cambridge Isotope Laboratories) or (iii) 5 mM glucose and 1 mM [U 13 C 15 N] glutamine

The pharmaceutical agent is injected slowly

NAC (2 mM, Sigma-Aldrich, A7250)

The risk is dose-dependent and increases with prolonged use, advanced age, concurrent corticosteroid or anticoagulant use, and history of peptic ulcer disease

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