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ERK1/2 phosphorylation displayed a biphasic kinetics showing a peak at 5 min, followed by a decline and a weaker second peak at 30 min in IR and IR/IGF1R-expressing cells (Supplementary Fig

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In the lysosomal lumen, arginine and leucine facilitate the activation of Ragulator by stimulating SLC38A, a sodium-coupled amino acid transporter and lysosomal V-ATPase on the lysosomal membrane, thereby promoting the binding of Rag GTPases and mTORC1 62,63,64

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