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Seeling et al., J Biol Chem 259: 9345

The prerequisite of the method is the genomic sequence of a phage which must be known to design expression vectors for bacterial production of tag-protein fusions

doi: 10.3390/microorganisms9122620

Spinal cord injury was induced in male Albino Wistar rats by controlled pressure (a vascular clip Aesulap 0.10-0.15 N) on the exposed spinal cord (at the level of Th12) for 30 seconds