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A 2017 review summarised neuroprotective properties of silymarin in animal models, including: Reduction of amyloidogenic activity (relevant in Alzheimer's research) Anti-inflammatory action in nerve tissue Reduction of oxidative stress in the brain Possible positive effects on cognitive function Possible reduction of stroke risk Whether these effects can be achieved in humans at attainable concentrations of silymarin in particular whether sufficient quantities can cross the blood-brain barrier remains unclear

Mixed with 500 L 70% EtOH and spun at 3000 g for 30 s at +4 C in a MiniElute column, washed with 350 L RW1, on-column digestion for 15 min at room temperature, washed with 350 L RW1, 500 L RLT, followed by 500 L 80% EtOH and spun for 2 min

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