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Following their respective differentiations, cells were cultured for 5 days in HPLM with sensitizing (0.1 nM) or resistance-inducing (3 nM) concentrations of insulin

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These include (1) tetrahydrobiopterin (BH 4 ), synthesized by the GTP cyclohydrolase 1 (GCH1)6-pyruvoyl-tetrahydropterin synthase (PTS)sepiapterin reductase (SPR) metabolic pathway and regenerated by dihydrofolate reductase (DHFR) 11