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Regulation and role of autophagy in mammalian cells

Liquid chromatography-mass spectrometry (LC-MS) analysis A LC-MS system consisting in a 1525 micro liquid chromatograph (Waters, Milford, MA, USA), a single wavelength UV-VIS detector Agilent 1100 (Agilent, Santa Clara, CA, USA) and a LCQ 3D-ion trap mass spectrometer (ThermoElectron, San Jose, CA, USA) equipped with an ElectroSpray Ionization (ESI) interface was used for the separation and analysis of standard vicine, faba bean flour extracts (naturally containing vicine and convicine), E-Ct (the mixtures resulting from the enzymatic hydrolysis of flour extract) and, finally, of extracts of faba bean flour doughs (FF, Ct and A-Ct)

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Conversely, adipocytes from obese mice express twofold lower levels of Gs, a subunit of the GTP-binding protein through which beta-adrenergic receptors stimulate adenylyl cyclase (38)