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2021;16:e0257070

Only one product is therefore needed to correct Vitamin B12 deficiency, regardless of the age or production stage of the animal, e.g

The soluble fraction was clarified by centrifugation (75,000 g , 20 min, 4 C) and incubated with 50% Ni-Sepharose suspension (GE Healthcare, Chalfont St Giles, UK) for 2 h at 4 C with gentle rotation prior to affinity purification