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Stomach acid and intestinal enzymes cleave the peptide into its component amino acids, which then need to be reassembled into glutathione inside cells a process that is inefficient and highly variable

Although siRNA studies show that GSTO1 is important for HCT116 cancer cell viability, knockdown of GSTO1 did not appear to affect C1-27 cytotoxicity, suggesting that other targets of C1-27 also may contribute to its cytotoxicity and have a compensatory effect (Supplementary Fig

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Acknowledgments We thank Lihong Shi and Jun Peng for critical reading of this paper