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To investigate, different temperature (RT, 28 C and 37 C) conditions were incubated for 24 h to simulate any possible oxidation that might occur during media preparation and in vitro or ex vivo experiments

To incubate DC2 cells with EV, the above protocol was followed with the exception that at the indicated times culture medium was supplemented with 3.14 10 8 EVs/mL in PBS, or an equal volume of PBS control for 3 days

An increase in TNF is linked to an increase in inflammation in the bodys digestive system - which can lead to a worsening of inflammatory bowel disease (IBD) symptoms

Identification of the target protein and molecular mechanism of honokiol in anti-inflammatory action

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