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Three independent points were selected in the renal cortex (1 mm below the capsule, targeting tubule-rich regions to enhance Ksp promoter-mediated specificity), with 1015 l virus (2.5 10 v.g.) injected slowly over 1015 s per point to avoid excessive pressure

The ultimate aim is to collect a representative portion of crops or fruits that can represent the whole batch or field ( 4.1.1 Sampling methods Different sampling methods are employed based on field size, crop type, and research objectives