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Upon TBZ-induced necroptosis, only the S309A mutant exhibited significantly impaired cell death, mirroring the phenotype observed in STK38-deficient cells (Fig

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Lagouge M, Argmann C, Gerhart-Hines Z, Meziane H, Lerin C, Daussin F, Messadeq N, Milne J, Lambert P, Elliott P, Geny B, Laakso M, Puigserver P, Auwerx J (2006) Resveratrol improves mitochondrial function and protects against metabolic disease by activating SIRT1 and PGC-1alpha

[Google Scholar] Liu, F., Cao, X.Z., Liu, Z.H., Guo, H., Ren, K.Q., Quan, M.F., Zhou, Y.F., Xiang, H.L., Cao, J.G., 2014

Beads were washed five times in LiCl wash buffer (50 mM HEPES pH 7.5, 500 mM LiCl, 1 mM EDTA, 1% NP-40, 0.7% sodium deoxycholate), once in TE/NaCl wash buffer (10 mM Tris pH 8, 1 mM EDTA, 50 mM NaCl) and proteinDNA conjugates eluted by incubating at 65 C in 50 mM Tris pH 8 with 10 mM EDTA and 1% SDS, followed by a further 6 h incubation at 65 C