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The crystallization was performed in Easy-Xtal 15-well crystallization plates by a hanging drop vapour diffusion, where 1 l of His-MBP-ApoE4 M was mixed with the reservoir solution (8AX9: 100 mM HEPES pH 7.5, 11% PEG 3350

Pediatric pulmonary hypertension: insulin-like growth factor-binding protein 2 is a novel marker associated with disease severity and survival

Therefore, the authors induced high expression of HA on the membrane of B16F10 cells through plasmid transfection