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For the biochemical and molecular analyses described below only the 20 M DPDS concentration was used, which resulted in four experimental groups: (1) Control DMSO vehicle, (2) 20 M DPDS, (3) 100 M TMZ, and (4) 20 M DPDS + 100 M TMZ

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Briefly, the protein-resin was packed into a gravity-flow column, washed with wash buffer (20 mM Tris-HCl, pH 7.5, 150 mM NaCl), and the protein was eluted with the same wash buffer containing 10 mM maltose