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Description

Heart J ., 9 (Suppl A), 95100

Step-by-Step Instructions

Berger MM

Lentiviral infection of IMR90 The viral supernatant for infection was obtained from the filtered growth media of the packaging cells HEK293T transfected with 3 g psPAX, 1 g pVSVG, 4 g of short hairpin RNA (shRNA) constructs and 24 l of Lipofectamine 2000 (Life Technology)