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Seeing the process in action can be an invaluable supplement to a written protocol

A randomized, double-blind, placebo-controlled, parallel, three-arm study was applied

Arginase-1, predominantly expressed in macrophages, and arginase-2, localized in various cells including myofibroblasts, both contribute to proline generation

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In a separate iteration of this experiment, HT-1080 cells were reverse-transfected with non-targeting (control) siRNAs or siRNAs targeting RRM1 and seeded as described above