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The culture medium was Dulbecco's Modified Eagle Medium (DMEM, D5796), supplemented with 10% fetal bovine serum (FBS, F7524), and 100 U/mL Penicillin-Streptomycin (P4333), referred to as complete DMEM. For HT29-MTX cells, 1% MEM Non-essential Amino Acid Solution (M7145,) was also added

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From an immunological perspective, these associations arise from a breakdown in self-tolerance mediated by overlapping T-cell and B-cell autoreactivity, shared cytokine pathways (e.g., IFN-, IL-18), and common antigen-presenting HLA alleles

nails may also break or crumble, or they may fall off at the nail bed About 30 percent of people with psoriasis will also suffer from psoriatic arthritis