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Lipid ROS levels were measured through oxidation of the Bodipy 581/591 C11 (#D3861, Invitrogen) according to the manufacturers instructions

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Kinetics comparisons of mammalian Atg4 homologues indicate selective preferences toward diverse Atg8 substrates

doi:10.3390/antiox10091473 Mignone LE, Wu T, Horowitz M, Rayner CK

Nevertheless, as discussed above, an abnormal postmethionine-load homocysteine elevation above fasting levels must be taken into account in the general identification of patients bearing transsulfuration defects, such as vitamin B 6 -deficient subjects or heterozygotes for CBS deficiency