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Cells were washed with ice-cold PBS, lysed in PBS containing 1% dodecyltrimethylammonium bromide in 0.2 M NaOH, and divided into two aliquotsone untreated and one treated with 1 M ascorbic acid followed by 0.4 M HCl

Although, HSE has also been reported to be a trigger of brain autoimmunity by detecting anti-N-methyl-D-aspartate (anti-NMDAR) antibodies in some patients after HSE disease (Armangue et al., 2014), the potential relationship between HSV-1 and autoimmunity in MS patients has been largely discussed and remains controversial, as some studies have shown contradictory results (Koros et al., 2014

[PubMed abstract] 39 Rimm EB, Willett WC, Hu FB, Sampson L, Colditz G A, Manson J E, Hennekens C, Stampfer M J

(HHV-6A was also active in the Hashimotos patients, but not in the healthy controls.) If HHV-6A is triggering Hashimotos, it might also be triggering the hypothyroidism that seems almost endemic in ME/CFS