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Approximately 3 mg of sample was loaded in a platinum pan and heated from 25 C to 200 C at a scanning rate of 10 C/min under a N 2 flow rate of 20 mL/min

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The supernatant was collected and set in a black 96-well plate for measurement of absorbance in a microplate reader (Biotek Synergy-HT, Winooski, VT, United States) at = 412 nm

Under some conditions, though, the body's need for glutathione is outpaced by its ability to produce it

Dorn C, Riener MO, Kirovski G, Saugspier M, Steib K, Weiss TS, Gabele E, Kristiansen G, Hartmann A, Hellerbrand C (2010) Expression of fatty acid synthase in nonalcoholic fatty liver disease