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The resulting supernatants were diluted 20-fold with PBS buffer before analysis using the established ratio fluorescence immunoassay method

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however, at pH 7.3, the proton concentration in the physicochemical microenvironment probably falls short to negate these charges

& Mori-Yoshimura, M.), 119 (Springer, 2015)

Mutations in the SNCA gene promote -syn aggregation into oligomers or fibrils, which disrupt the ubiquitin-proteasome system and impair membrane binding by reducing its N-terminal -helical structure, leading to compromised membrane integrity and increased permeability (141, 142)