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RB: Methodology, Investigation

Following that, the tissue samples were immersed in graded concentrations of sucrose buffer (1030%) for cryoprotection at 4 C overnight

Acknowledgements: CRAM:Research Ireland:13/RC/2073_P2,SENSOME Disclosure of interest: All authors:nothing to disclose 1 - ICH & SAH 02.00 - INTRACRANIAL HEMORRHAGE - 02.01 - INTRACEREBRAL HAEMORRHAGE P740 - ESOC25-498 HEMATOMA LOCATION AND SYMPTOM ONSET TO SAMPLING ARE PREDICTORS OF GFAP RELEASE INTO PLASMA IN ACUTE INTRACEREBRAL HEMORRHAGE Sabina Zylyftari 1 , Love-Preet Kalra 1 , Sebastian Luger 1 , Stephan Barthelmes 1 , Kristaps Blums 1 , Christian Foerch 1 1 RKH Klinikum Ludwigsburg, Ludwigsburg, Germany Background and Aims: GFAP (glial fibrillary acidic protein) is a promising biomarker for distinguishing intracerebral hemorrhage (ICH) from ischemic stroke in the prehospital phase

While the FDA is not requiring that these new forms be included with submissions immediately, we highly recommend that sponsors adopt them as soon as possible