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The reactions were carried out in 384 well PCR plates in a total volume of 4 l containing 10 M of reverse and forward primers, 2 l of SensiFAST Sybr lo ROX RT-PCR mix (Bioline, Boston, MA), and 5 ng of template cDNA (1 l)

Large cohorts show that deficiency is more common at diagnosis of stomach and colorectal cancers, likely because these tumours interfere with intrinsic-factor secretion or gut absorption

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established a modified DTT technique that requires lower DTT concentrations, is less time-consuming, and appears to avoid denaturation of Kell antigen [52]

** Based on the treatment regimen estimand: treatment effect regardless of whether patients stayed on treatment or took other glucose-lowering therapies