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3bf) Solutions of cysteine, glutathione or histidine or a mixture of these compounds were prepared in PBS, and a solution of 13 C-formaldehyde was added, so that the final concentration of these compounds (or their mixture) was 2.5 mM, and with this twice the concentration of formaldehyde that was used with 1.25 mM, as this better reflects the situation in vivo, in which the concentration of formaldehyde should be lower than that of the amino acid traps

In Norway, Sweden, and Russia this limit was moved even lower to 0.02% [5]