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6D12 at 1:1000), washed with PBS-T, and then incubated at room temperature for 1 h with a peroxidase-conjugated goat anti-mouse IgG antibody (1:25,000)

However, during myogenesis, GW182 detaches and HDAC4 loses 5cap and poly(A) tail, suggesting that loss of GW182 alone or in combination with changes in HDAC4 facilitates the transport of Ago2: miR-1 into mitochondria ( Figure 8A )

Luczkowska et al., 2022)

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