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The antagonism of acetylcholine can lead to adverse reactions such as cognitive impairment, confusion, blurred vision, hallucinations, urinary retention, and constipation

Fully differentiated HepaRG cells (Lonza, catalog: NSHPRG) were plated with 90% of Williams E Medium (Thermo Fisher Scientific, catalog: A1217601) and supplemented with 10% HepaRG thawing and plating medium supplement (Lonza, catalog: MHTAP) and 25 U/mL penicillin-25 g/mL streptomycin