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The GGT-shRNA-Lentivirus was diluted in DMEM with 10% FBS and supplemented with polybrene at a final concentration of 5 g/mL to enhance transduction efficiency

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Because autophagy may serve as a protective mechanism for CLL cells (36), we then tested CB-839 in combination with the autophagy inhibitor chloroquine at a concentration of 50 M ( Supplemental Figure 5 )

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