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Afterward, the sections were incubated for an additional two hours at room temperature with fluorophore-conjugated secondary antibodies: goat anti-rabbit IgG (Alexa Fluor 594 conjugate, Invitrogen, #A11008, 1:1000) and goat anti-mouse IgG (Alexa Fluor 594 conjugate, Invitrogen, #A21422, 1:1000)

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