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Take precautions to maintain uniformity of sterilizing and cooling conditions throughout the assay, since packing tubes too closely in the autoclave, or overloading it, may cause variation in the heating rate

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23: 414424, doi: Di Marzio L, Moretti S, DAlo S, Zazzeroni F, Marcellini S, Smacchia C, Alesse E, Cifone MG, De Simone C

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