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Olive oil, a staple of the Mediterranean diet, is rich in monounsaturated fatty acids (particularly oleic acid), antioxidants, and phenolic compounds, all of which contribute to its therapeutic properties against NAFLD

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Larger, randomized controlled trials (RCTs) with longer follow-up periods are necessary to evaluate the efficacy and safety of topical glutathione more robustly

For method 2 sample preparation, the initial sample preparation was the same as described for method 1, except that NEM was added to the extraction solvent (50 L of 50 mM NEM in 80% methanol, 20% 10 mM ammonium formate, pH 7.0 and 50 L of 100% ACN)

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