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2.10 Analysis of DIA-based quantitative proteomics 2.10.1 Protein extraction Liver samples were first grinded by liquid nitrogen and then the powder was transferred to a 1.5 mL centrifuge tube and sonicated three times on ice, using a high intensity ultrasonic processor in a lysis buffer (8M urea including 1mM PMSF, 2 mM EDTA)