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We prepared HEK293T cells expressing cell surface-targeted LaccID, fixed the cells and overlaid them with DAB

Eur J Pharmacol 583(1):7383 di Maio R, Mastroberardino PG, Hu X et al (2013) Thiol oxidation and altered NR2B/NMDA receptor functions in in vitro and in vivo pilocarpine models: implications for epileptogenesis [J]

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doi: 10.1073/pnas.1318431110 147

In their case they calculated the average number of mutations per culture 'from the proportion of cultures containing no resistant bacteria at the moment of the test' in accordance with Poisson's law (Luria, Delbrck)