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Antibiotics may contribute to oxidative stress by increasing reactive oxygen species (ROS), aligning with observations of elevated ROS levels in ME/CFS patients

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Liver enzymatic activity assay The liver tissue was homogenized and tissue myeloperoxidase (MPO), malonaldehyde (MDA), GSH, glutathione peroxidase (GSH-px) activities were measured using the activity assay kits from NanJing JianCheng Bioengineering Institute according to the manufacturer's instructions

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Ichinohe, T., Kawaguchi, A., Tamura, S.-I., Takahashi, H., Sawa, H., Ninomiya, A., Imai, M., Itamura, Sh., Odagiri, T., Tashiro, M., Chiba, J., Sata, T., Kurata, T., and Hasegawa, H., Intranasal immunization with H5N1 vaccine plus Poly I:Poly C12U, a Toll-like receptor agonist, protects mice against homologous and heterologous virus challenge, Microbes Infect